Protocols

This section provides protocols for MicrobeTracker use in order to perform a few most typical tasks. For a particular operation, the user may also need to read the description of the mentioned operation (button or tool) on their corresponding help pages.

See also available related tutorials that guide you through some of the mentioned operations on the example of provoded image sets:

Usage for independent images

You do not have to save the results during processing. However, this is preferable for large image sets in case the program crashes. In addition, there are memory leaks in MATLAB that cannot be easily eliminated, so that during long processing the memory requirements grow. If you are processing large image sets, it may be preferable to do the detection without fluorescence signal. In this case restart MATLAB after detection, load the phase contrast, the fluorescence images and the analysis, then subtract the background, check Reuse, and click All frames to add the signal without cells detection. In addition, if you have noticed excessive memory held by MATLAB in Windows task manager after running for a long time, restarting MATLAB may also be advisable.

Usage for timelapses

Usage for moving cells

If the cells are moving very little, the timelapse regime may still work. You can also increase moveall parameter to ~ 0.95. In most cases, however, you should follow the procedure below.

 

Top pages: MicrobeTracker, MicrobeTracker Suite.